| 产克拉维酸的棒状链霉菌突变株的选育 |
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| 引用本文： | 王艳萍,张永生,张阳,左志晗.产克拉维酸的棒状链霉菌突变株的选育[J].食品与发酵工业,2007,33(5):9-12. |
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| 作者姓名： | 王艳萍 张永生 张阳 左志晗 |
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| 作者单位： | 天津市食品营养与安全重点实验室,天津科技大学食品工程与生物技术学院,天津,300457 |
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| 摘 要： | 以棒状链霉菌(Streptomyces clavuligerus),B71为出发菌株,通过紫外线诱变育种,采用琼脂块法初筛和摇瓶发酵复筛,选育出2株甘油耐受性正向突变株S.clavuligerus B71—14和S.clavuligerus B71-49,在摇瓶条件下,其克拉维酸产量与出发菌株S.davuligerus B71相比,分别提高了90.6%和88.8%,并具有良好的遗传稳定性。
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| 关 键 词： | 棒状链霉菌 紫外诱变 耐甘油 克拉维酸 |
| 修稿时间： | 2006-10-182007-01-25 |
Breeding of Streptomyces clavuligerus Mutant with Glycerol Resistance |
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| Wang Yanping,Zhang Yongsheng,Zhang Yang,Zuo Zhihan.Breeding of Streptomyces clavuligerus Mutant with Glycerol Resistance[J].Food and Fermentation Industries,2007,33(5):9-12. |
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| Authors: | Wang Yanping Zhang Yongsheng Zhang Yang Zuo Zhihan |
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| Affiliation: | Tianjin The Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457,China |
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| Abstract: | The original strain Streptomyces clavuligerus B71 was treated with UV light, and methods including an agar column solid fermentation model and the shake-flask model were used for primary and further selection. Finally, S. clavuligerus B71-14 and S. clavuligerus B71--49 with high glycerol-resistant were ob- tained and their clavulanic acid yield increased 90.6% and 88.8% compared with S. clavuligerus B71. This character can be inherited stably. |
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| Keywords: | Streptomyces clavuligerus ultraviolet mutagenesis glycerol-resistant clavulanic acid |
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