Non-destructive Determination of High Oleic Acid Content in Single Soybean Seeds by Near Infrared Reflectance Spectroscopy |
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Authors: | Sang-Ik Han Jong-Hyun Chae Kristin Bilyeu J Grover Shannon Jeong-Dong Lee |
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Affiliation: | 1. Department of Functional Crop, Functional Crop Resource Development Division, RDA, Milyang, 627-803, Republic of Korea 4. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea 2. Plant Genetic Research Unit, USDA-ARS, 110 Waters Hall, Columbia, MO, 65211, USA 3. Division of Plant Sciences, University of Missouri-Delta Center, Portageville, MO, 63873, USA
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Abstract: | Soybean Glycine max (L.) Merr] with increased oleic acid is desirable to improve oxidative stability and functionality of soybean seed oil. Recently, soybean genotypes with high oleic acid (≥70 %) were developed by breeding programs. Efficient and effective identification of high oleic acid soybean genotypes using non-destructive near infrared reflectance (NIR) on whole seeds would greatly enhance progress in breeding programs. The objective of this study was to develop a calibration equation for NIR determination of high oleic acid from single soybean seeds. A total of 600 intact, single F2 seeds were scanned by NIR. Spectral data were collected between 400 and 2,500 nm at 2 nm intervals. The relationship between NIR spectral patterns of each soybean seed and its oleic acid content was examined. The best predicted equations for oleic acid were selected on the basis of minimizing the standard error of cross-validation and increasing the coefficient of determination. Validation demonstrated that the equations for determining total oleic acid and over 50 % oleic acid content had high predictive ability (r 2 = 0.91 and r 2 = 0.99, respectively). To validate the newly developed equation, F2 seeds from a different genetic background were tested. Again, high oleic acid from single soybean seeds was accurately predicted from various genetic backgrounds. Therefore, applying the calibration equations to NIR will be useful to rapidly and efficiently select high oleic acid soybean genotypes in breeding programs. |
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Keywords: | Soybean Oleic acid Near infrared reflectance Single intact seed |
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