高效液相色谱法测定番茄与土壤中双胍三辛烷苯基磺酸盐残留量

建立了一种用高效液相色谱测定番茄与土壤中双胍三辛烷苯基磺酸盐的定量分析方法。番茄样品用正丁醇和正己烷提取，然后加三乙胺溶液进行液液分配，经硅胶柱层析净化；土壤样品用氢氧化钠-甲醇溶液提取，然后加三氯甲烷进行液液分配，经硅胶柱层析净化。甲醇-水-氨水作流动相，荧光检测器检测，外标法定量。番茄添加水平为0．2～5．0mg／kg时，回收率为70％-88％，变异系数2．1％～8．7％；土壤添加水平为0．2～5．0mg／kg时，回收率为62％-95％，变异系数3．3％-4．9％；对番茄和土壤的最低检出浓度分别是0．05、0．10mg／kg。

HPLC studies on quantitative determination of iminoctadine tris(albesilate) in tomato and soil

A HPLC method for the determination of iminoctadine tris(albesilate) residue in tomato and soil was established.Tomato were extracted with n-butanol and n-hexane, then liquid-liquid partitioned with triethylamine solution, and cleaned up through silica gel. Soil were extracted with sodium hydroxide- methanol, then liquid-liquid partitioned with chloroform, and cleaned up through silica gel. Samples were detected with fluoroscope, the mobile phase was methanol -water and ammonia solution. Iminoctadine tris(albesilate) was added with 0.2 to 5.0mg/kg in spacing tomato, the average recoveries ranged from 70 to 88%. The RSD was 2.1 to 8.7% iminoctadine tris(albesilate) was added with 0.2 to 5.0mg/kg in spacing soil, the average recoveries ranged from 62 to 95%. The RSD was 3.3 to 4.9%. The detection limits of the method were 0.05, 0.10mg/kg.