A Chamber for Testing the Release of Volatile Substances Secreted by Animals,Especially Mammals,as Exemplified by Substances Released by Rats in Response to Stress |
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| Authors: | Prof Roman Hauser Marcin Marczak Jacek Namie?nik Bartosz Karaszewski |
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| Affiliation: | 1. Department of Forensic Medicine , Medical University of Gdańsk , Poland rohauser@amg.gda.pl;3. Department of Analytical Chemistry , Technical University of Gdańsk , Poland;4. Department of Neurology of Adult , Medical University of Gdańsk , Poland |
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| Abstract: | Abstract The success of the testing of volatile organic compounds emitted by animals is dependent on the creation of appropriate conditions for air sampling subsequently used to assay and identify the compounds. These conditions play a particularly important role in the investigation of pheromones, which are secreted in extremely low concentrations. The authors have not come across any previous work which offers constructional solutions, which would allow avoidance of contamination of the air samples containing volatile substances secreted by animals. A constructional solution was developed, which provides optimal conditions for their sampling and isolation. Its main advantages are as follows: the exposure chamber (the chamber in which the animal is studied) is filled with synthetic air; the exposure chamber is separated from the atmospheric air with a synthetic air “jacket”; the exposure chamber has been constructed using materials which do not release chemicals and absorb them in trace quantities. |
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| Keywords: | Experimental animals Stress conditions Exposure chamber Pheromones Alarm pheromones Sampling Isolation of analytes |
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