新型自组装纳米胶团人工过氧化物酶研究 |
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引用本文: | 杨天,;杨晓璐,;张钰帅,;耿方勇,;康慧茹,;肖保林,;洪军,;穆萨维姆瓦赫迪.新型自组装纳米胶团人工过氧化物酶研究[J].广州化工,2014(16):34-37. |
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作者姓名: | 杨天 ;杨晓璐 ;张钰帅 ;耿方勇 ;康慧茹 ;肖保林 ;洪军 ;穆萨维姆瓦赫迪 |
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作者单位: | [1]河南大学生命科学学院,河南开封475000; [2]河南大学生物工程研究所,河南开封475000; [3]伊朗德黑兰大学生物化学与生物物理研究所,伊朗德黑兰 |
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基金项目: | 河南省科技发展计划项目(No.142300410124);河南大学特聘教授科研启动资金(No.5443D08010)和伊朗国家科学基金(INSF). |
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摘 要: | 十二烷基肌氨酸钠与天然细胞色素c通过自组装方式构建了一种新型纳米胶团结构人工过氧化物酶(Artificial Peroxidase,AP)。采用紫外可见光分光光度计对AP酶促反应过程进行研究。表明AP在较宽的pH范围具有活性,并在pH 5.0,100 mmol/L磷酸盐缓冲液中达到最大。该AP的米氏常数Km、催化速率kcat以及催化效率分别为1.70μmol/L、0.11 s-1、0.065μM-1s-1,催化效率为天然辣根过氧化物酶的90.2%。
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关 键 词: | 细胞色素c 人工过氧化物酶 十二烷基肌氨酸钠纳米胶团 催化效率 |
Research on a New Self-Assembly Nano Micelles Artifical Peroxidase |
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Affiliation: | YANG Tian, YANG Xiao-lu, ZHANG Yu-shuai, GENG Fang-yong, KANG Hui-ru, XIAO Bao-lin, HONG Jun, MOOSAVI-MOVAHEDI Ali Akbar ( 1 School of life sciences, Henan University, Henan Kaifeng 475000 ; 2 Institute of Biotechnology, Henan University, Henan Kaifeng 475000, China; 3 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran) |
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Abstract: | A nanostructured artificial peroxidase ( AP ) was constructed self -assembly using cytochrome c and sodium N-lauroyl sarcosine nano miceller.UV-vis spectroscopic study showed that the AP had peroxidase activity in a wide pH range with maximum peroxidase activity at pH 5.0.The Michaelis -Menten (Km), catalytic rate (kcat) and catalytic efficiency were obtained to be 1.70 μmol/L, 0.11 s-1 , 0.065 μM-1 s-1 , respectively.The catalytic efficiency of the AP was 90.2%as efficient as the native horseradish peroxidase ( HRP). |
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Keywords: | cytochrome c artificial peroxidase sodium N -lauroyl sarcosine nano micellar catalytic efficiency |
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