KMnO_4-荧光素化学发光体系的研究

甲睾酮与浓硫酸的反应产物在酸性高锰酸钾反应产生弱的化学发光。基于荧光素能使显著增强体系的化学发光强度,建立了测定甲睾酮的化学发光新方法。该方法的线性范围分别为8.0×10-8~1.0×10-5 g/mL,检出限为3.0×10-8。对6.0×10-6 g/mL甲睾酮溶液进行11次平行测定,其相对标准偏差为2.6%。该方法己应用于片剂中甲睾酮的含量测定,结果满意。

Study on the Chemiluminescence System of KMnO4-fluorescein

It’s found that the weak chemiluminescence can be produced during the reaction of methyltestosterone with sulfuric acid. Based on the condition that fluorescein can make the system of chemiluminescence be intensity, a new method to determine chemiluminescence was established. The linear range of the method was 8.0×10^-8-1.0×10^-5 g/mL.The detection limits was 3.0×10^-8 g/mL. The relative standard deviation was 2.6%.The method can be applied to determine methyltestosterone in table with satisfactory result.