| 反相高效液相色谱法测定川楝子中阿魏酸含量 |
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| 引用本文： | 杨冠华,李阳,张丽,许良.反相高效液相色谱法测定川楝子中阿魏酸含量[J].广州化工,2011(3):11-12,15. |
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| 作者姓名： | 杨冠华 李阳 张丽 许良 |
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| 作者单位： | 内蒙古民族大学化学工学院,内蒙古,通辽,028042 |
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| 摘 要： | 建立测定川楝子中阿魏酸含量的高效液相色谱法。采用VP-ODS C18（250 mm×4.6 mm,5μm）色谱分析柱;流动相为甲醇-1%冰醋酸（25：75）;检测波长：320 nm;流速：0.8 mL/min。结果阿魏酸进样量在0.25~2.50μg范围内与峰面积呈良好的线性关系（r=0.999 6）,平均加样回收率为99.38%,RSD=1.18%（n=6）。该方法简便、准确、可靠,可作为川楝子中阿魏酸的定量分析方法。
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| 关 键 词： | HPLC 川楝子 阿魏酸 |
Determination of Ferulic Acid in Melia Toosendan by RP-HPLC |
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| YANG Guan-hua,LI Yang,Zhang Li,XU Liang.Determination of Ferulic Acid in Melia Toosendan by RP-HPLC[J].GuangZhou Chemical Industry and Technology,2011(3):11-12,15. |
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| Authors: | YANG Guan-hua LI Yang Zhang Li XU Liang |
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| Affiliation: | (Chemical & Engineering College,Inner Mongolia University for the Nationalities,Inner Mongolia Tongliao 028042,China) |
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| Abstract: | HPLC method for the determination of ferulic acid in Melia toosendan was established.The chromatographic separation was performed on a VP-ODS C18 column(250 mm×4.6 mm,5 μm),using methanol-1.0% glacial acetic acid(25:75) as mobile phase.The detection wavelength set at 320 nm and the flow rate was 0.8 mL/min.The results showed that the linear range of ferulic acid was 0.25~2.50 μg,the average recovery rate was 99.38%(r=0.999 6),and RSD was 1.18%(n=6).This method was simple,accurate and reliable.It could be used as the method to determine the contents of ferulic acid in Melia toosendan. |
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| Keywords: | HPLC |
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