牛磺酸对顺铂引起的小鼠肾髓质ATP酶活性变化的影响

目的 :探讨牛磺酸对顺铂引起的小鼠肾髓质ATP酶活性的影响 ;方法 :4 0只小鼠随机分为正常对照组、CDDP组、牛磺酸 2 0 0mg组 (Tau2 0 0 )和牛磺酸 4 0 0mg组 (Tau4 0 0 ) ,分取肾髓质、制备匀浆 ,用生化法测定Na ,K -ATP酶、Ca2 -ATP酶和Mg2 -ATP酶活性 ;结果 :CDDP组、Tau2 0 0组、Tau4 0 0组肾髓质Na ,K -ATP酶、Ca2 -ATP酶和Mg2 -ATP酶活性均明显低于正常对照组 (P <0 .0 1)。Tau2 0 0组、Tau4 0 0组肾髓质Na ,K -ATP酶和Ca2 -ATP酶活性均明显高于CDDP组 (P <0 .0 1或P <0 .0 5 ) ,而Mg2 -ATP酶活性与CDDP组相比无显著差别 (P >0 .0 5 ) ;结论 :牛磺酸可明显增加肾髓质Na ,K -ATP酶和Ca2 -ATP酶活性 ,对顺铂引起的肾毒性具有防治作用。

Influence of taurine on the changes of ATPases activity induced by cisplatin in renal medulla of mice

Objective:To explore the influences of taurine on the changes of ATP ases activity induced by cisplatin in renal medulla of mice.Methods:40 mice were randomly divided into four groups such as control group,CDDP group,Tau 200 group and Tau 400 group,and the tissue of renal medulla was isolated and the activities of Na ,K -ATPase ,Ca 2 -ATPase and Mg 2 -ATPase were assayed by biochemistry.Results:The activities of Na ,K -ATPase,Ca 2 -ATPase and Mg 2 -ATPase were significantly decreased in CDDP group,Tau 200group and Tau 400 group when comparing with control group( P <0.01),the activities of Na ,K -ATP ase and Ca 2 -ATP ase in Tau 200 group and Tau 400 group were higher than it in CDDP group( P <0.01 or P <0.05),but the activities of Mg 2 -ATPase in Tau 200 group and Tau 400 group were no different when comparing with CDDP group( P >0.05).Conclusion:Taurine might have the preventive effect on cisplatin induced-nephrotoxicity by obviously increasing the activities of Na ,K -ATPase and Ca 2 -ATPase in renal medulla tissue of mice.