板栗壳色素化学性质及结构的初步研究

以板栗壳为材料,首先优化了板栗壳色素的纯化方法,在此基础上研究了纯化色素的化学性质、紫外-可见光谱及红外光谱。硅胶柱层析表明,优化的色素纯化方法简便有效。紫外-可见光谱分析表明:(1)色素甲醇溶液在紫外光区有两个明显的吸收峰,分别在218nm和264nm附近;(2)pH3.0、4.0条件下,紫外光区在218nm和264nm附近有明显的吸收峰,可见光区在480nm附近有小的吸收峰;(3)pH5.0、6.0条件下,紫外光区在218nm和272nm附近有明显的吸收峰,可见光区在510nm附近有小的吸收峰,中性及碱性条件下的光谱也具有相同特点。红外光谱分析表明,色素的主要吸收波数为3400、3145、3043、1715、1630、1400、1036和620cm-1。结合色素的化学性质鉴定,初步推断板栗壳色素含有苯环及酚羟基,而不具有典型黄酮类色素的结构。

Preliminary Study on Chemical Properties and Molecular Structure of Pigment from Chestnut Shells

This experiment optimized the purification method of pigment from chestnut shells,and then analyzed the chemical properties,ultraviolet(UV) spectrum and infrared(IR) spectrum. The results of silica gel column chromatography indicated that the new method is simple and effective. The UV spectrum analysis showed that:(1) In methanol solution,there are strong absorption peaks near 218 nm and weak absorption peak near 264 nm.(2) In pH 3.0 or 4.0 NaOH-HCl solution,there are apparent absorption peaks near 218 nm and 264 nm and small absorption peak near 480 nm.(3) In pH 5.0 or 6.0 NaOH-HCl solution,there are apparent absorption peaks near 218 nm and 272 nm and small absorption peak near 510 nm,so does the UV spectrum in alkaline solution. IR spectrum revealed that the main absorption wave numbers of the pigment are 3400,3145,3043,1715,1630,1400,1036 and 620 cm-1. According to the results of chemical property analysis,it was concluded preliminarily that there are benzene ring and phenolic hydroxyl,but no tipical flavonoids structure existing in the chemical structure of the pigment.