| 牛血清白蛋白的荧光稳定性研究 |
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| 引用本文： | 陈芳,陈妍,张彦峥,张银堂,徐茂田.牛血清白蛋白的荧光稳定性研究[J].应用化工,2011(9). |
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| 作者姓名： | 陈芳 陈妍 张彦峥 张银堂 徐茂田 |
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| 作者单位： | 商丘师范学院化学系纳米生物分析化学河南省高校重点实验室培育基地;贵州大学精细化工研究开发中心;郑州大学化学系; |
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| 基金项目： | 国家自然科学基金项目(20905045); 河南省科技厅科技攻关项目(102102310385); 河南省教育厅自然科学基础研究计划项目(2009B150021); 商丘师范学院青年科学基金项目资助(2009QN09) |
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| 摘 要： | 研究了牛血清白蛋白(BSA)在不同条件下荧光发射光谱的稳定性。结果表明,Tris-HCl、PBS和PB等缓冲液种类,常量和微量等石英比色皿类型,对于BSA的荧光稳定性没有明显影响。激发波长260 nm与280 nm对于BSA的荧光稳定性有显著性差异。超声和金属离子的干扰,都会导致BSA荧光强度的降低,在5%的误差范围内,各金属离子允许存在的倍数分别为:Cu2+(8),Zn2+(40),Mg2+(96),Ca2+(120)。本研究对于蛋白质溶液的制备和前处理具有一定的理论和实际意义。
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| 关 键 词： | 牛血清白蛋白 荧光 稳定性 缓冲液 超声 |
Fluorescence stability of bovine serum albumin solutions |
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| CHEN Fang,CHEN Yan,ZHANG Yan-zheng,ZHANG Yin-tang,XU Mao-tian.Fluorescence stability of bovine serum albumin solutions[J].Applied chemical industry,2011(9). |
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| Authors: | CHEN Fang CHEN Yan ZHANG Yan-zheng ZHANG Yin-tang XU Mao-tian |
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| Affiliation: | CHEN Fang1,CHEN Yan2,ZHANG Yan-zheng3,ZHANG Yin-tang1,XU Mao-tian1,3 (1.Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry,Department of Chemistry,Shangqiu Normal University,Shangqiu 476000,China,2.Center for Research and Development of Fine Chemicals,Guizhou University,Guiyang 550025,3.Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | Fluorescence stability of bovine serum albumin(BSA) solutions was investigated under different conditions.The results indicated that buffer solutions,e.g.Tris-HCl,PBS and PB,and quartz cuvettes such as macro-and micro-cuvette did not affect fluorescence stability of BSA.BSA fluorescence showed significant stability diversity when excitation wavelength of 260 nm and 280 nm were used.Fluorescence intensity of BSA may be lowered by ultrasound or metal ions.Within 5% tolerance range of the detection value,the m... |
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| Keywords: | bovine serum albumin fluorescence spectroscopy stability buffer solution ultrasound |
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