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细菌抑制剂离体生物活性试验
引用本文:陈亮,刘君丽,司乃国.细菌抑制剂离体生物活性试验[J].农药,2012,51(7):533-535.
作者姓名:陈亮  刘君丽  司乃国
作者单位:沈阳化工研究院有限公司新农药创制与开发国家重点实验室,沈阳,110021
基金项目:国家“十二·五”科技支撑计划,农药生物活性筛选技术研究
摘    要:目的]为明确化学药剂对病原细菌的离体抑菌活性,进而为田间病害防治提供优秀的候选药剂。方法]将黄瓜细菌性角斑病菌(Pseudomonas syringae pv.lachrymans)等常见病原细菌作为防治靶标,利用带菌平板法,对药剂的离体抑菌活性进行对比研究。结果]不同的药剂对病原细菌的离体抑菌活性差异很大,链霉素、土霉素等药剂对多种病原细菌具有较高的离体抑菌活性。结论]试验采用带菌平板法,能够对药剂的离体抑菌活性进行准确检测。

关 键 词:杀细菌剂  生物活性  离体试验

The Research of Bactericide Bioactivity in vitro
CHEN Liang , LIU Jun-li , SI Nai-guo.The Research of Bactericide Bioactivity in vitro[J].Pesticides,2012,51(7):533-535.
Authors:CHEN Liang  LIU Jun-li  SI Nai-guo
Affiliation:(State Key Laboratory of the Discovery and Development of Novel Pesticide,Shenyang Research Institute of Chemical Industry Co.,Ltd.,Shenyang 110021,China)
Abstract:For the purpose of control the bacterial disease with bactericide,we carry out experiment in vitro.Methods] Agrobacterium vitis,Erwinia carotovora subsp.carotovora,Pseudomonas syringae pv.lachrymans et al.as control target,make use of germ carried flat method,inspect the bioactivity of bactericide.Results] Different bactericide have different bioactivity against pathogenic bacteria.In these bactericide,streptomycin and oxytetracycline have the best bioactivity than others.Conclusions] With the method of germ carried flat,we can determine the bioactivity of bactericide in vitro.
Keywords:bactericide  bioactivity  experiment in vitro
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