Biomemory device composed of mutant azurin thin films modified by site-directed mutagenesis |
| |
| Authors: | Sang-Uk Kim Ajay Kumar Yagati Junhong Min Jeong-Woo Choi |
| |
| Affiliation: | aInterdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea;bCollege of Bionanotechnology, Kyungwon University, Bokjung-dong, Sujung-gu, Seongnam 461-701, Republic of Korea;cDepartment of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea |
| |
| Abstract: | A bioelectronic device composed of self-assembled mutant azurin thin films with memory function has been developed for molecular electronics. Azurin was recombined with a cysteine residue to enhance the stability of self-assembled protein on gold surface. The thin films of protein on gold substrate were investigated by surface plasmon resonance spectroscopy, scanning tunneling microscopy and Raman spectroscopy, respectively. The memory characteristics, including the “read”, “write” and “erase” functions of self-assembled azurin layer, were obtained with three distinct electrical states of azurin layers by cyclic voltammetry. These results show the proposed biomemory device as a step towards the protein based nanobiochip. |
| |
| Keywords: | Bioelectronic device Site-directed mutagenesis Surface plasmon resonance Scanning tunneling microscopy Raman spectroscopy Cyclic voltammetry Nanobiochip |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
