高效液相色谱法测定苹果果实中的有机酸

目的:建立苹果果实中有机酸含量的测定方法。方法:采用高效液相色谱法(high performance liquidchromatography,HPLC),水浴超声提取果实中的有机酸,色谱条件:色谱柱为WatersWAT010290(7.8mm×300mm,7μm),流动相为0.01mol/L H2SO4溶液,流速0.5mL/min,柱温50℃,检测波长210nm。结果:在选定的色谱条件下,柠檬酸、酒石酸、苹果酸、琥珀酸、乙酸和草酸都得到很好分离。6种有机酸的线性范围为0.001～1.000(草酸为0.800)mg/mL,标准曲线相关系数均在0.9998以上;精密度检测,RSD为0.10%～1.59%(n=5);重现性检测,RSD为1.79%～4.26%(n=5);回收率在91.05%～105.18%之间。结论:该方法简单快捷、准确、重复性好,可用于苹果果实中的有机酸测定。

Determination of Organic Acids in Apple Fruits by HPLC

Objective: To establish a method for determining organic acids in apple fruits by high performance liquid chromatography(HPLC).Methods: Organic acids were extracted from apple fruits by ultrasonic-assisted water extraction.An IC PAKTMION Exclusion(Waters,WAT010290) column was used to separate organic acids.The mobile phase was 0.01 mol/L H2SO4 with a flow rate of 0.5 mL/min.The column temperature and UV detection wavelength were set as 50 ℃ and 210 nm,respectively.Results: Under chosen experimental conditions,six organic acids could be successfully separated.All the calibration curves for these organic acids displayed a good linear relationship with correlation coefficients above 0.9998 and precision RSDs of 0.10%-1.59%(n = 5) and repeatability RSDs of 1.79%-4.26%(n = 5).The recovery rates of six organic acids were in the range of 91.05%-105.18%.Conclusion: The method is convenient,rapid,accurate and applicable to determine organic acids in apple fruits.