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| 应用MTT比色法测定EPO体外生物学活性 | |
| 引用本文： | 周勇 张燕军. 应用MTT比色法测定EPO体外生物学活性[J]. 中国生物制品学杂志, 1995, 8(3): 122-123,139 |
| 作者姓名： | 周勇 张燕军 |
| 作者单位： | 中国药品生物制品检定所!北京，100050，中国药品生物制品检定所!北京，100050，中国药品生物制品检定所!北京，100050，中国药品生物制品检定所!北京，100050，中国药品生物制品检定所!北京，100050 |
| 摘 要： | EPO依赖细胞珠在EPO浓度梯度中培养72小时，再加入MTT培养5小时，离心弃上清后，加入有机溶剂溶解MTT代谢产物，以OD值对EPO浓度梯度作量效曲线，定量地测定EPO生物学活性。本法重复性好，与其它体外生物学活性测定方法所测定结果相符。 |
| 关 键 词： | 红细胞生成素 MTT 生物学活性 |
Determination of Biological Activity of EPO In Vitro by MTT Colorimetric Method |
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| Zhou Yong et aI. Determination of Biological Activity of EPO In Vitro by MTT Colorimetric Method[J]. Chinese Journal of Bilogicals, 1995, 8(3): 122-123,139 | |
| Authors: | Zhou Yong et aI |
| Abstract: | EPO dependent cell strains cultured in EPO concentration gradient for 72hours were continuously culttired for 5 hours after MTT were added. The MTT metabolicproducts were separated by centrifugation and dissolved by organic solvents, then detectedbyspectrophotometry. Dose-effect graph was made by plotting EPO concetration against ODvalue. and used for the quantitative determination of the biological activity of EPO. Themethod showed good reproducibility compared with other methods. |
| Keywords: | EPO MTT Biological activity |
| 本文献已被 CNKI 维普 等数据库收录！ | |