| HPLC测定健脾止泻宁颗粒中黄芩苷含量 |
| |
| 引用本文： | 母小东,陈晓虎,王慧.HPLC测定健脾止泻宁颗粒中黄芩苷含量[J].山东食品科技,2012(11):432-434. |
| |
| 作者姓名： | 母小东 陈晓虎 王慧 |
| |
| 作者单位： | [1]武警四川省消防总队医院,四川成都610071 [2]重庆市食品药品检验所,重庆401121 |
| |
| 摘 要： | 目的建立高效液相色谱法测定健脾止泻宁颗粒中黄芩苷含量的方法。方法采用Agilent C18色谱柱（4．6mm×150mm，5μm），甲醇-0．4％磷酸溶液（50：50）为流动性，检测波长280nm。结果黄芩苷进样量在0．02114～0．5285μg（r=0．9999）线性关系良好；平均回收率100．8％，RSD=0．9％。结论本方法准确、简便，可用于健脾止泻宁颗粒的质量控制。
|
| 关 键 词： | 健脾止泻宁颗粒 黄芩苷 高效液相色谱法 |
Determination of the Baicalin in Jianpi Zhixieling Granules by HPLC |
| |
| Authors: | MU Xiao-dong CHEN Xiao-hu WANG Hui |
| |
| Affiliation: | 1. Sichuan Police Hospital of Fire Control Army Unit, Chengdu 610071, China; 2. Chongqing Institute for Food and Drug Control, Chongqing 401121, China) |
| |
| Abstract: | Objective To establish an HPLC method for the quantitative determination of baicalin in Jianpi Zhixieling granules. Methods The analysis was carried out on Agilent C18 column （4.6 mm×150 mm, 5 μm） with methanol-0.4 % phosphoric acid solution （50：50） as mobile phase, and the detection wavelength was 280 nm. Results Linearity of baicalin was good （r=0.9999） in ranges of 0.02114-0.5285 μg. The average recovery was 100.8 % with RSD of 0.9 % （n=6）, respectively. Conclusion The method is simple and accurate, and can be used for the quality control of Jianpi Zhixieling granules. |
| |
| Keywords: | Jianpi Zhixieling granules baicalin HPLC |
| 本文献已被 维普 等数据库收录！ |
