Dis-SPE-UPLC-MS/MS测定卷烟主流烟气中4种TSNAs

烟草特有N-亚硝胺（TSNAs）是卷烟烟气中重要的致癌成分，为准确测定卷烟主流烟气中的TSNAs释放量，建立了UPLC-MS/MS同时测定卷烟主流烟气中4种TSNAs的检测方法。采用剑桥滤片捕集卷烟主流烟气，经醋酸铵水溶液提取，N-丙基乙二胺（PSA）净化后UPLC-MS/MS测定。结果显示，TSNAs的工作曲线在1~110 ng/mL之间线性关系良好（R²>0.99），检出限（LOD，以S/N=3计）0.008~0.016 ng/mL；回收率82.5%~94.8%，精密度4.9%~9.8%。该方法与标准方法进行比对效果良好，相关系数的范围为0.9991~0.9995。该方法准确可靠、选择性好，适合于烤烟型卷烟主流烟气中TSNAs释放量的检测。

Determination of Four Tobacco-specific N-nitrosamines in Tobacco Mainstream Smoke by Dispersive Solid Phase Extraction-UPLC-MS/MS

Tobacco-specific nitrosamines (TSNAs) are very important carcinogens in tobacco and cigarette smoke. In order to develop a robust analytical method to determine low levels of TSNAs in cigarette smoke, a dispersive solid phase extraction-UPLC-MS/MS method was used to simultaneously determine four TSNAs in mainstream cigarette smoke. Mainstream smoke was collected on a cambridge filter pad, further extracted with ammonium acetate aqueous solution, then cleaned up by primary secondary amine (PSA), and subsequently analyzed with UPLC-MS/MS. The proposed method displayed limit of detections between 0.008 and 0.016 ng/mL and good linearity over the range from 1 to 110 ng/mL, with a R²>0.99. Recovery varied from 82.5% to 94.8% for Chinese Virginia cigarettes. Mean precisions ranged from 4.9%-9.8%. The proposed method, which provides higher sensitivity and specificity, is suitable for quantitatively analyzing TSNAs yield in Chinese Virginia cigarettes.