| 基于纳米金和辣根过氧化物酶信号放大的
癌胚抗原电化学免疫传感器 |
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| 引用本文： | 杨高建,赖玉璇,汤从利,邓 燕.基于纳米金和辣根过氧化物酶信号放大的
癌胚抗原电化学免疫传感器[J].湖南工业大学学报,2018,32(5):27-31. |
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| 作者姓名： | 杨高建 赖玉璇 汤从利 邓 燕 |
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| 作者单位： | 湖南工业大学生物医用纳米材料及器件湖南省重点实验室 |
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| 摘 要： | 以癌胚抗原(CEA)为检测目标构建CEA电化学免疫传感器:壳聚糖为生物活性膜,纳米金和生物素标记的辣根过氧化物酶为放大元,CEA、链霉亲和素标记的癌胚抗体免疫反应形成的复合物为固定的酶标免疫复合物,并对检测条件进行了优化。通过竞争性酶联免疫法检测不同浓度CEA的电化学响应。结果表明,该新型免疫传感器表现出良好的线性范围(4～16 ng/mL,R2=0.992 7),检测限为1 ng/mL。
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| 关 键 词： | 电化学免疫传感器 酶联免疫 癌胚抗原（CEA） 纳米金（AuNPs） |
| 收稿时间： | 2017/11/27 0:00:00 |
A Carinembryonic Antigen Electrochemical Immunosensor Based on
Gold Nanoparticles and Horseradish Peroxidase Signal Amplification |
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| YANG Gaojian,LAI Yuxuan,TANG Congli and DENG Yan.A Carinembryonic Antigen Electrochemical Immunosensor Based on
Gold Nanoparticles and Horseradish Peroxidase Signal Amplification[J].Journal of Hnnnan University of Technology,2018,32(5):27-31. |
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| Authors: | YANG Gaojian LAI Yuxuan TANG Congli and DENG Yan |
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| Abstract: | A novel cancerembryo antigen (CEA) electrochemical immunosensor has been constructed using chitosan (CS) as bioactive membranes, gold nanoparticles (AuNPs) and biotinylated horseradish peroxidase (HRP) for signal amplification, and CEA, streptavidin-labeled carcinoembryonic anti-bodies (anti-CEA) immunoreactive complexes as immobilized enzyme-labeled immunoconjugates, accompanied by the optimization of the detection conditions. The effect of different electrochemical responses at different concentrations of CEA can be detected by adopting the competitive enzyme linked immunoabsorbent assay ( ELISA). This novel sensor exhibits such fine properties as with a good linear range (4~16 ng/mL, R2=0.992 7) and a low detection limits of 1 ng/mL. |
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