Diffuse reflectance fourier transform infrared spectroscopy of phospholipid adsorption onto silicic acid |
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Authors: | C Adhikari A Proctor G D Blyholder |
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Affiliation: | (1) Department of Food Science, University of Arkansas, 272 Young Ave., 72704 Fayetteville, AR;(2) Department of Chemistry and Biochemistry, University of Arkansas, 72704 Fayetteville, Arkansas |
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Abstract: | Diffuse reflectance Fourier transform infrared spectroscopy was used to study the mode of adsorption of phosphatidylcholine
(PC) in hexane onto silicic acid (SA). PC adsorption was mainly through the charged phosphate group with minimal binding through
the ester carbonyl. When the SA surface with adsorbed PC is washed with hexane, containing a small concentration of isopropanol,
the desorbed PC is recovered without structural change, i.e., there is no evidence of PC hydrolysis in the adsorption process.
Adsorbent misture probably promotes PC adsorption due to the increased availability of surface water hydroxyl groups for interaction
with the PC phosphate groups. Isopropanol promoted PC binding by destabilizing PC reverse miscelles in solution, thus promoting
its adsorption. |
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Keywords: | Adsorption Fourier transform infrared spectroscopy isopropanol phosphatidylcholine phospholipid water |
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