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| 柳蒿芽多酚的提取纯化及其抗氧化活性 | |
| 引用本文： | 崔艳艳,柳佳莹,董然,沈明浩. 柳蒿芽多酚的提取纯化及其抗氧化活性[J]. 粮食与油脂, 2019, 32(9): 76-80 |
| 作者姓名： | 崔艳艳 柳佳莹 董然 沈明浩 |
| 作者单位： | 通化师范学院食品科学与工程学院,吉林通化,130000;吉林农业大学食品科学与工程学院,吉林长春,130118 |
| 摘 要： | 以柳蒿芽为原料,在单因素试验的基础上通过Plackett-Burman试验和Box-Behnken试验确定最优提取方案,并利用D101大孔吸附树脂进行纯化,最终得到柳蒿芽多酚。结果表明:柳蒿芽多酚的最佳提取工艺为乙醇浓度70%、酶解温度60℃、酶解pH 5.5、加酶量50 mg/g、酶解时间2 h、料液比1∶30(g/mL)、超声时间30 min、超声功率400 W,在此条件下,柳蒿芽多酚得率为9.41%,纯化后的柳蒿芽多酚纯度为76.2%。体外抗氧化试验结果表明,柳蒿芽多酚质量浓度为1.2 mg/mL时,Fe3+还原力和DPPH+清除力最强,分别为1.09和94.20%,且高于维生素C对照组,说明柳蒿芽多酚抗氧化活性较强。 |
| 关 键 词： | 柳蒿芽多酚 提取 纤维素酶 响应面 |
Extraction and purification of polyphenols from Artemisia integrifolia and its antioxidant activity |
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| CUI Yan-yan,LIU Jia-ying,DONG Ran,SHEN Ming-hao. Extraction and purification of polyphenols from Artemisia integrifolia and its antioxidant activity[J]. Cereals & Oils, 2019, 32(9): 76-80 | |
| Authors: | CUI Yan-yan LIU Jia-ying DONG Ran SHEN Ming-hao |
| Affiliation: | (College of Food Science and Engineering,Tonghua Normal University,Tonghua 130000,Jilin,China;College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,Jilin,China) |
| Abstract: | CUI Yan-yan;LIU Jia-ying;DONG Ran;SHEN Ming-hao(College of Food Science and Engineering,Tonghua Normal University,Tonghua 130000,Jilin,China;College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,Jilin,China) |
| Keywords: | Artemisia integrifolia L. polyphenol extraction cellulase response surface |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |