| 小麦麸皮中戊聚糖的纯化分级及组成分析 |
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| 引用本文： | 郑学玲,姚惠源.小麦麸皮中戊聚糖的纯化分级及组成分析[J].食品与生物技术学报,2002,21(4):332-335. |
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| 作者姓名： | 郑学玲 姚惠源 |
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| 作者单位： | 江南大学,食品学院,江苏,无锡,214036 |
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| 基金项目： | 国家“十五”重点科技攻关计划项目 (2 0 0 1BA50 1A0 4 )资助课题 |
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| 摘 要： | 用乙醇逐步沉淀和离子交换柱层析方法对麸皮中制备的水溶戊聚糖(WSP)和水不溶戊聚糖 (AEP)进行分级纯化 ,并对各组分的组成进行了分析 .结果表明 ,用乙醇逐步沉淀时 ,随着乙醇体积分数的增加 ,所得分级组分具有较高的分支 ;用离子交换柱层析时 ,WSP可分级为 2个组分 ,AEP可分级为 3个组分 ,并且用NaCl较H2 O洗脱的组分具有较高的分支和较高的相对分子质量
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| 关 键 词： | 小麦麸皮 水溶戊聚糖 水不溶戊聚糖 分级 |
| 文章编号： | 1009-038X(2002)04-0332-04 |
| 修稿时间： | 2001年12月18 |
The Purification and Composition Analysis of Pentosans from Wheat Bran |
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| ZHENG Xue ling,YAO Hui yuan.The Purification and Composition Analysis of Pentosans from Wheat Bran[J].Journal of Food Science and Biotechnology,2002,21(4):332-335. |
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| Authors: | ZHENG Xue ling YAO Hui yuan |
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| Abstract: | In order to obtain homogenous fractions, water soluble pentosans(WSP) and water insoluble pentosans(AEP) prepared from wheat bran were fractionated by stepwise ethanol precipitation and DEAE cellulose column chromatograph. The composition of each pentosan was analyzed. The results showed that with the gradual increase of ethanol concentration, Ara/Xyl ratio of obtained fractions from WSP and AEP was raised,indicating a high branch degree of pantosans. WSP were purified into two fractions, and AEP were purified into three fractions upon DEAE cellulose column chromatography. The fractions eluted with NaCl had higher branch degree and higher molecular weight than the fractions eluted with water. |
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| Keywords: | wheat bran water soluble pentosan water insoluble pentosan fractionation |
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