煤化工烟道气毒性成分对Chlorella pyrenoidosa生长和细胞成分的影响

探究煤化工烟道气中毒性成分对微藻的影响是利用微藻固定煤化工烟道气CO₂实现减排的关键。本文利用不同浓度的NaHS、Na₂SO₃和NH₃·H₂O培养Chlorella pyrenoidosa（C. pyrenoidosa），以探究煤化工烟道气主要毒性成分H₂S、SO₂和NH₃气体水溶物的毒性。实验结果表明：NaHS、Na₂SO₃和NH₃·H₂O浓度分别低于1mmol/(L·d)、40mmol/(L·d)和7mmol/(L·d)时对C. pyrenoidosa生长无抑制作用，而且Na₂SO₃＜40mmol/(L·d)]会显著促进 C. pyrenoidosa的生长；NaHS 添加4mmol/(L·d)时会在生长初期抑制C. pyrenoidosa的生长，NH₃·H₂O添加35mmol/(L·d)则会直接造成藻细胞的破碎死亡。与对照组相比，NaHS和Na₂SO₃浓度分别低于1mmol/(L·d)、10mmol/(L·d)时对C. pyrenoidosa的细胞成分无影响；NaHS添加4mmol/(L·d)使藻蛋白含量提高7.13%；Na₂SO₃添加40mmol/(L·d)使藻蛋白降低13.45%，总糖含量提高42.90%；NH₃·H₂O的添加会使藻蛋白含量降低，总糖含量提高。微藻生物质整体蛋白质含量较高，可作为蛋白饲料来源。研究结果表明，C. pyrenoidosa对煤化工烟道气中的主要毒性气体有较好的耐受性，利用煤化工烟道气培养微藻具有可行性。

Effects of toxic components of flue gas from coal chemical industry on growth and cell components of Chlorella pyrenoidosa

Investigating the resistance of microalgae to harmful gases in coal chemical flue gas is the key to reduce CO₂ emission by microalgae fixation. NaHS, Na₂SO₃, and NH₃·H₂O were used as the solubles of H₂S, SO₂, and NH₃ to simulate these three typical harmful gases in coal chemical flue gas, and different concentrations were added into the medium of microalgae C. pyrenoidosa to clarify the effects on the physiology and biochemistry of algae cell. Results showed that there were no inhibitory effects on the growth of C. pyrenoidosa when NaHS, Na₂SO₃ and NH₃-H₂O were added below 1mmol/(L·d), 40mmol/(L·d), and 7mmol/(L·d), respectively. Na₂SO₃ ＜40mmol/(L·d)] could significantly promote the growth of C. pyrenoidosa, but NaHS added 4mmol/(L·d) inhibited the growth of C. pyrenoidosa at the initial stage of algae growth, and NH₃·H₂O added 35mmol/(L·d) directly caused the death of algae cells. Compared with control group, there were no effects on the cell components of C. pyrenoidosa while NaHS and Na₂SO₃ were added below 1mmol/(L·d) and 10mmol/(L·d), respectively. However, NaHS added 4mmol/(L·d) increased protein content of microalgae by 7.13%. Na₂SO₃ added 40mmol/(L·d) reduced the protein content by 13.45%, and increased the total sugar content by 42.90%. Overall, microalgae biomass had high protein contents with a potential use of protein feed source. This study demonstrates that C. pyrenoidosa has high tolerance to H₂S, SO₂ and NH₃. Thus, it is feasible to cultivate microalgae using coal chemical flue gas.