| 电位滴定法测定苦瓜中总生物碱的含量 |
| |
| 引用本文： | 石雪萍,汪洪涛,余芳,陈成. 电位滴定法测定苦瓜中总生物碱的含量[J]. 食品与发酵工业, 2013, 39(5): 190-193 |
| |
| 作者姓名： | 石雪萍 汪洪涛 余芳 陈成 |
| |
| 作者单位： | 江苏经贸职业技术学院工程技术学院,江苏省食品安全工程技术研究开发中心,江苏南京,210007 |
| |
| 基金项目： | 江苏经贸职业技术学院院级重大课题 |
| |
| 摘 要： | 苦瓜分别经酸水提取、乙醇回流提取、超声波提取和碱浸提取,得到的样品以电位滴定法滴定,以苦瓜中的奎宁为基准物计算苦瓜中总生物碱的含量,由此建立苦瓜中总生物碱的含量测定方法。结果表明:电位滴定法具有良好稳定性、灵敏度和重现性,检测结果稳定可靠,且测定操作简便,可用于苦瓜中总生物碱的定量分析。四种提取方法得到生物碱的含量分别为4.644、4.428、6.804和8.964 mg/g,适宜的提取方法为碱浸提取法。
|
| 关 键 词： | 苦瓜 生物碱 电位滴定 |
Determination of total alkaloids in Momordica charantia L by potentiometric titration |
| |
| SHI Xue-ping,WANG Hong-tao,YU Fang,CHEN Cheng. Determination of total alkaloids in Momordica charantia L by potentiometric titration[J]. Food and Fermentation Industries, 2013, 39(5): 190-193 |
| |
| Authors: | SHI Xue-ping WANG Hong-tao YU Fang CHEN Cheng |
| |
| Affiliation: | (Department of Engineering and Technology of Jiangsu institute of economic & trade technology, Jiangsu food Safety Engineerning Research and Development center Nanjing,Jiangsu 210007) |
| |
| Abstract: | Dried Momordica charantia L were extracted through HCl-water,ethanol refluxing,ultrasonic extract,and alkaline leaching-chloroform extraction.The alkaloids were calculated by reference material.The results showed that potentiometric titration method had good stability and high sensitivity and high reliability.It could be used as a quantitative analysis of total alkaloids in Momordica charantia L.Total alkaloids contents of the Momordica charantia L with four extracting methods were 4.644 mg/g,4.428 mg/g,6.804 mg/g and 8.964mg/g respectively.The optimal extracting method was alkaline leaching-chloroform extraction. |
| |
| Keywords: | Momordical charantia L alkaloids potentiometric titration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
