自然感染鼠痘的小鼠肝脾内病毒超微结构的观察

应用自然感染鼠痘存档5年蜡块转制超薄切片，在电镜下观察发现鼠痘病毒的复制部位是在肝脾细胞浆的基质区和包涵体的周围。此部位有大量不同发育阶段的病毒颗粒。未成熟病毒颗粒可分为球状颗粒和含有拟核的球状颗粒。成熟病毒颗粒的典型形态呈年椭圆形，外被双层囊膜，中心含有哑铃形核心，核心两侧各有一个测体。本实验结果证实应用存档蜡块转制超薄切片，可成功地应用于鼠痘感染及追溯性鼠痘病原和传染源的电镜诊断。并在电镜下证实光镜下A、B型包涵体及免疫酶组化病毒抗原的阳性部位与鼠痘病毒生物合成和装配的部位是一致的。

Ultrastructure of Poxivirus in Naturally Infected Murine Liver and Spleen

After the paraffin blocks of liver and spleen of mice naturally infected with poxvirus had been stored for 5 years, the ultrathin sections of them were observed under electron microscope. The result showed that the replication sites of poxvirus were at matrical aeras of hepato-lienal cytoplasma and periphery of in inclusion bodies. Massive virus particles at various developmental stages were found in the sites. Immature virus were spherical particles with uniform viroplasm or nucleoid. Mature virus were oval particals consisted of a dumb-bell-shaped core and two lateral bodies and enveloped with bilaminar cyst membrane.The result proved that the ultrathin sections of stored paraffin blocks could be successfully used for diagnosis of mlce pox infection with electron rnicroscope, and retrospective diagnosis of pathogeny and source of infection of mice pox as well. Electron mlcroscope examination further demonstrated that the sltes where inclusion bodies (type A and B) and immunoenzyme histochemistry virus antigens, which had been detected out by optical microscope,were coincided with that where pox virus were synthesized and assembled.