静电纺再生丝素纳米纤维的结构与性能

静电纺丝获得的丝素纳米级纤维可作为细胞培养支架,用于纺丝工艺及后处理能改变丝素微细结构,影响其水溶性和力学性能。本文采用XRD、FTIR、固态13CNMR和DSC研究了不同工艺下丝素纳米纤维及经甲醇处理后的微细结构,比较了不同微细结构下的水溶性和力学性能。结果表明,电纺丝的微细结构受纺丝工艺影响,高电压、纺丝液中丝素质量分数大时纺得的电纺丝结晶度高,经甲醇处理后,β化程度提高;w(丝素)=11%、15%时制备的电纺丝断裂强度分别为8.5、11.9 cN/mm;w(丝素)=11%、19%,水溶性由51.2%下降到43.3%;w(丝素)=19%、电压32 kV制得的电纺丝甲醇处理前后水溶性从43.3%下降到6.6%,说明丝素纳米纤维结晶度提高,强度增加、水溶性下降,满足了细胞支架的要求。

Structure and Properties of Electrospun Regenerated Silk Fibroin Nanofibers

The electrospun silk fibroin nanofibers can be usd as cell scaffold,while the spinning process and the aftertreatment change the microstructure and affect its water-solubility and mechanical performance.This paper researched the microstructure of electrospun silk fibroin nanofibers under different process conditions and with methanol treatment by means of XRD,FTIR,solid state ~(13)CNMR and DSC,and studied their water-solubility and mechanical performance.The results show that the process parameters affect the electrospun fibers′ microstructure,large concentration and high voltage lead to high crystallinity,and methanol treatment brings about more β-sheet structure.Break strengths of the electrospun fibers are 8.5 and 11.9 cN/mm respectively at different concentrations of 11% and 15%,and the water-solubility at concentrations of 11% and 19% decreased from 51.2% to 43.3%.But under the condition of 19% and 32 kV,the value of water-solubility decreased to 6.6% from original 43.3% after methanol treatment,indicating the increase of crystallinity and strength and the decrease of water-solubility of the electrospun silk fibroin nanofibers,conforming to the requirement of cell scaffold.