固相萃取-高效液相色谱法测定猪肉与鸡肉中替米考星、泰乐菌素残留量

建立了高效液相色谱法(HPLC)测定猪肉与鸡肉中替米考星、泰乐菌素残留量的检测方法.样品用乙腈提取,C18固相萃取小柱净化,采用高效液相色谱分离,二极管阵列检测器(PDA)检测,外标法定量.结果表明:标准校准曲线在30～1000μg/L的范围内呈良好线性关系,相关系数r＞0.999.样品中替米考星、泰乐菌素的添加水平分别为0.05、0.10、0.15mg/kg时,样品加标回收率为72.7％～90.8％,变异系数在2.6％～8.3％之间,替米考星和泰乐菌素方法检出限30μg/kg,定量限为50μg/kg.本法简便、快速、定量准确、精密度高,适用于猪肉和鸡肉中替米考星与泰乐菌素残留量的检测.

Determination of Tilmicosin and Tylosin Residues in Pork and Chicken by SPE-HPLC

An SPE-HPLC method to determine tilmicosin and tylosin residues in pork and chicken has been established.Samples were extracted with acetonitrile and extracted antibiotic residues were cleaned up using a C18 SPE column,separated by HPLC,detected with a PDA detector and quantified by external standard method.The established calibration curve showed good linearity in the range of 30 to 1000 μg/L(r>0.999).The recovery rates of tilmicosin and tylosin from samples spiked at concentrations of 0.05,0.10 mg/kg and 0.15 mg/kg were 72.7%–90.8%,with coefficients of variation of 2.6%–8.3%.The limits of detection(LODs) and limits of quantification(LOQs) for both antibiotics were 30 μg/kg and 50 μg/kg,respectively.The HPLC method was simple,rapid,accurate,precise and suitable to determine tilmicosin and tylosin residues in pork and chicken.