海狗油中脂肪酸的组成成分

研究了两种不同的衍生化方法对海狗油中脂肪酸成分分析的影响．分别用杂环化法和甲酯化法衍生化海狗油，应用气相色谱-质谱联用法对海狗油其中含有的脂肪酸成分进行分析，结果发现：用杂环化法衍生化海狗油，再经GC／MS分析可分离鉴定出30个峰，30种成分，并能对长链多不饱和脂肪酸的双键位置进行准确地定位；用甲酯化法衍生化海狗油，再经GC／MS分析只能分离出25个峰，15种成分得到鉴定(有的峰为同一种成分)，有两个峰未确定，且双键数目相同而位置不同的脂肪酸的色谱峰分离不够理想．总体而言，杂环化衍生化法优于甲酯化衍生化法．

The Analysis and Study of Fatty Acid Components in Seal Oil

Seal oil was derivated with heterocycliczation method and methyl esterization method respectively, and analyzed with GC/MS. The result showed that, Ge profill had 30 peaks, 30 compounds were indentified, and the double bonds in long-chain polyunsaturated fatty acids could be accuratoly loeated, under the case of derivating seal oil with heterocycliczation method. On the other hand, when using methyl esterization method, the GC profile showed 25 peaks, 15 compounds were indentified, same numbers of chromatogram peaks for double bond fatty acids were found but with different position and bad isolation. In summary, heterocycliczation method is better than methyl esterization method.