荧光染色法观察LED活化竹红菌素介导的光动力作用诱导卵巢癌细胞凋亡

目的:探讨LED活化竹红菌素介导的光动力作用诱导人卵巢癌细胞凋亡的光化学生物学效应.方法:应用Hoechst33258细胞核荧光染色法观察LED活化竹红菌素介导的光动力作用对人卵巢癌细胞凋亡的影响,结果:LED活化竹红菌素介导的光动力作用组出现核凝聚、凋亡小体的人卵巢癌细胞株H08910细胞明显多于单纯LED辐照组、单...

Apoptosis induced by LED- activated Hypocerllin B in human ovariancarcinoma cells using fluorescent staining analysis

Objective： To observe the photochemical biological effect of Lighting Emitting Diode（LED） - activated Hypocerllin B on the apoptosis of ovarian carcinoma cells. Methods： A special lighting emitting diode equipment was employed to investigate the photochemical biological effect of Hypocerllin B 4h after photedynamic therapy in a human ovarian carcinoma cell line H08910 by Hoechst33258 fluorescent staining analysis. Results： Hoechst33258 staining showed that the morphological changes in the nuclear ehromatin of LED- activated Hyvx~rllin B in H08910 were extraordinarily siofificant.ln the control groups, the nuclei were stained a less bright blue and the color was homogeneous. After being irradiated by LED with Hypocernin B for 4h, the blue emission light in apoptotic cells was much bright than the control cells. Condensation of ehromatin and nuclear fragmentations could also be found in many treated cells and some of them formed the structure of apoptotic bodies, which is one of the classic characteristics of apoptotie cells. Conclusion： Lighting Emitting Diode （LED） - activated Hypocerllin B could effectively induce apoptosis of human ovarian carcinoma cells and Lighting Emitting Diode（LED） will become an alternative light source for photedynamic therapy.