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| 实时荧光PCR定性定量检测混合食用油脂中的花生油成分 | |
| 引用本文： | 覃文,董洁,邓鸿铃.实时荧光PCR定性定量检测混合食用油脂中的花生油成分[J].中国油脂,2006,31(10):73-76. |
| 作者姓名： | 覃文 董洁 邓鸿铃 |
| 作者单位： | 1. 广东化工制药职工技术学院,510520 2. 广东出入境检验检疫局,510623,广州市 3. 广州市产品质量监督检验所,510110,广州市 |
| 基金项目： | 广东省科技厅社会发展攻关项目(编号:C32204)的部分研究内容 |
| 摘 要： | 为鉴别鉴定花生油在食用油脂中的存在与否及其含量,研究了定性定量检测花生特异性基因片段的分子生物学检测方法。结果表明,生花生需加热处理后再提取DNA才能作为PCR扩增的模板,食用油脂中可提取出用于定性定量PCR的DNA,花生的Arah1基因是具有花生种属特异性的基因,采用实时荧光PCR方法检测Arah1基因片段可定性定量检测混合食用油脂中的花生油成分。 |
| 关 键 词： | 花生油 种属特异性基因 定性定量PCR |
| 文章编号： | 1003-7969(2006)10-0073-04 |
| 收稿时间： | 2006-04-18 |
| 修稿时间： | 2006年4月18日 |
Qualitative and quantitative detection of peanut oil in blend oil by real-time PCR |
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| QIN Wen,DONG Jie,DENG Hong-ling.Qualitative and quantitative detection of peanut oil in blend oil by real-time PCR[J].China Oils and Fats,2006,31(10):73-76. | |
| Authors: | QIN Wen DONG Jie DENG Hong-ling |
| Affiliation: | 1. Guangdong Vocational College of Pharmaceutics,510520 Guangzhou, China; 2. Guangdong CIQ,510623 Guangzhou, China; 3. Guangzhou Products Quality Supervision and Testing Institute, 510110 Guangzhou, China |
| Abstract: | The identification and quantification of peanut oil in blend oil by using real-time PCR method was studied.Primers and Taqman probe was designed to detect Arachis hypogaea major allergen Arah1 used as endogenous gene of peanuts in the assay.The results showed that DNA directly from the crude peanut and its derived oil were not suitable for PCR.The extracted DNA from the crude peanut and its derived oil which were heated could be employed as the template of PCR.Arah1 gene was detected and it differentiated peanut from the other oil seads.With real-time PCR method and specific primers and probe,the quantitative detection for the components of peanut oil in blend oil can be performed. |
| Keywords: | peanut oil detection real-time PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |