烤烟中硝酸盐和亚硝酸盐的测定

本文提出了一种使用紫外分光光度计测定硝酸盐和亚硝酸盐的方法。主要过程包括将烟样在弱碱性条件下热水中萃取,亚硝酸盐标准曲线的绘制,亚硝酸盐含量的测定,硝酸盐的还原,硝酸盐含量的测定,回收率的测定。该方法具有简单、高效、重复性好、不需专用仪器设备等特点,适用于各种烟草样品中硝酸盐和亚硝酸盐含量的测定。 

The quantification of nitrite and nitrate in flue-cured tobacco

Nitrate and nitrite are important nitrogen compounds in tobacco. It has been always difficult to quantify them accurately at high speed. An analytical procedure for quantification of nitrate and nitrite in tobacco was developed using ultraviolet spectrophotometer. The procedure involves the extraction by hot water from tobacco in basic property condition. Standard curve of nitrite was drawn, quantification of nitrite, reduction of nitrate to nitrate, quantification of nitrate, recovery for the quantification of nitrate and nitrite was determined. This method is shown to be simple in determination of nitrate and nitrite in various tobacco samples with acceptable repeatability.