| Abstract: | The degradation of several azo dyes by the cell-free extract from Pseudomonas stutzeri has been studied. The compounds 2-carboxy-4′-dimethylaminoazobenzene, 4-carboxy-4′-dimethylaminoazobenzene, 2-carboxy 4′-die thy lam inoazobenzene, 2-met hoxycarbonyl-4′-dime t h y lam inoazobenzene, 4-dime t h y lam inoazobenzene sulphonic acid, and 2-hydroxy-1 -naphthaleneazobenzenesulphonic acid were appreciably degraded by the cell- f ree extract. The cell- f ree extract was quite specific for 2-ca rboxy-4 ′-diet h y lam inoazo benzen e, 2 met hoxyca rbonyI4′-dime t h y lam inoazo benzene, and 2-ca rboxy4′-dime t h y lam inoazo benzene, which a reequal in their redox potential. Thus, it is suggested that the substrate specificity is characterised by a log Pdependence. The presence of azo reductase in the cell-free extract was alsoconfirmed by electrophoresis. |
