Lactobacillus buchneri IMAU80233高密度发酵工艺优化

以工业化生产为出发点,对具有潜在益生特性Lactobacillus buchneri IMAU80233高密度培养工艺进行优化。采用Bioscreen C读值系统,在MRS培养基的基础上,对适合L. buchneri IMAU80233生长增殖培养基成分进行快速筛选优化。优化后最适培养基为果糖80 g/L,大豆蛋白胨23.90 g/L,酵母粉11.90 g/L,柠檬酸1.59 g/L,柠檬酸钠20.06 g/L,MnSO_4·5H_2O 0.09 g/L,MgSO_4·7H_2O 0.60 g/L,精氨酸0.50 g/L,吐温-80 1.00 g/L。采用5 L×3联发酵罐进行小试,确定初始pH值为6.5,37℃恒定pH 5.9培养20 h,发酵初期通入氮气,优化后发酵液活菌数达3.81×10~9 CFU/mL。

Optimization of High Cell Density Culture of Lactobacillus buchneri IMAU80233

The high cell density culture of Lactobacillus buchneri IMAU80233 with potential probiotic characteristics was optimized for industrial production in this study. MRS medium was improved and rapid selection and optimization of suitable medium components for the growth and proliferation of this strain were carried out using Bioscreen C system. The optimal medium composition was determined as follows: fructose 80 g/L, soybean peptone 23.90 g/L, yeast powder 11.90 g/L, citric acid 1.59 g/L, sodium citrate 20.06 g/L, MnSO4·5H2O 0.09 g/L, MgSO4·7H2O 0.60 g/L, arginine 0.50 g/L, and Tween-80 1.00 g/L. Five-liter fermenters (× 3) were used to carry out small-scale experiments. The initial pH value was 6.5, and IMAU80233 was cultured at 37 ℃ for 20 h during which the pH was held constant at 5.9. Nitrogen gas was introduced during the initial fermentation stage. Under the optimized conditions, the viable cell count of the fermentation broth was 3.81 × 109 CFU/mL.