Poly(ethylene glycol) decorated poly(methylmethacrylate) nanoparticles for protein adsorption |
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| Authors: | Alfredo Bonfá Rafael S.N. Saito,Rafael F.O. Franç aBenedito A.L. Fonseca,Denise F.S. Petri |
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| Affiliation: | a Instituto de Química, Universidade de São Paulo, P.O. Box 26077, 05513-970 São Paulo, Brazilb Programa de Pós Graduação em Imunologia Básica e Aplicada, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazilc Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil |
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| Abstract: | Poly(ethylene glycol) decorated poly(methyl methacrylate) particles were synthesized by means of emulsion polymerization using poly(ethylene glycol) sorbitan monolaurate (Tween-20) as surfactant. PMMA/PEG particles presented mean diameter (195 ± 15) nm, indicating narrow size distribution. The adsorption behavior of bovine serum albumin (BSA) and concanavalin A (ConA) onto PMMA/PEG particles was investigated by means of spectrophotometry. Adsorption isotherms obtained for BSA onto PMMA/PEG particles fitted well sigmoidal function, which is typical for multilayer adsorption. Con A adsorbed irreversibly onto PMMA/PEG particles. The efficiency of ConA covered particles to induce dengue virus quick agglutination was evaluated. |
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| Keywords: | Poly(ethylene)glycol Nanoparticles BSA Concanavalin A Dengue virus Adsorption |
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