Two-stage photoautotrophic cultivation to improve carbohydrate production in Chlamydomonas reinhardtii |
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| Affiliation: | 1. Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA;2. Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6120, USA;3. Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA;4. School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA;5. National Renewable Energy Laboratory, Golden, CO 80401, USA;1. Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China;2. State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China;3. School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia;1. FAPESP, Instituto Oceanográfico da Universidade de São Paulo, Praça do Oceanográfico, 191, CEP 05508-900 São Paulo, SP, Brazil;2. CIETI/ISEP, School of Engineering, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida 431, 4249-015 Porto, Portugal;3. LEPABE/FEUP, Faculty of Engineering of University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal |
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| Abstract: | A two-stage strategy was developed to improve the microalgal carbohydrate accumulation for advanced biofuel production. In the first stage, Chlamydomonas reinhardtii CC125 was cultivated in photo-bioreactors by repeated fed batch operation to improve the biomass production. Optimal culture conditions achieved in the batch operation were applied to the repeated fed batch operation. Biomass productivity reached 0.47 g L?1 d?1 with 70% medium replacement ratio and 5% CO2 under continuous light of 135 μmol m?2 s?1. In the second stage, reducing CO2 content to 0.04% led to a high carbohydrate content of 71%, showing more than 9 times improvement compared to that in the biomass from the first stage culture. These results suggest that photoautotrophic two-stage cultivation is an effective approach to accumulate microalgal carbohydrate as a feedstock for biofuel production. |
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| Keywords: | Microalgae Carbohydrate Productivity Carbon dioxide |
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