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Standardised Mangifera indica extract is an ideal antioxidant |
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| Authors: | Lai Teng Ling Su-Ann Yap Ammu K Radhakrishnan Thavamanithevi Subramaniam Hwee Ming Cheng Uma D Palanisamy |
| Affiliation: | 1. Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Department of Pathology, Faculty of Medicine and Health, International Medical University, No. 126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia;3. Bio-Cosmetic and Natural Products Programme, SIRIM Berhad, 40911 Shah Alam, Selangor, Malaysia;4. School of Medicine and Health Sciences, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia |
| Abstract: | The standardised ethanolic and aqueous extracts of Mangifera indica leaf were prepared and analysed for their free radicals scavenging activity. The IC50 values using the DPPH assay were 0.17 ± 0.02 and 0.49 ± 0.4 mg/ml, respectively. Standardised ethanolic extracts of the M. indica leaf had a solid content of 9.1 ± 0.7%, mangiferin concentration of 73 ± 0.17 mg/g of dry weight of the extract, free radical scavenging activity (IC50) of 0.17 ± 0.02 mg/ml and total phenolic content of 590 ± 48 mg/g of extract. The protection exhibited by these extracts against lipid peroxidation was superior to butylated hydroxytoluene (BHT) and commercial grape seed extract. These extracts at higher concentration did not exhibit pro-oxidant activities when compared to vitamin C. Our findings also show that the aqueous and ethanolic extracts of M. indica leaf protect NIH/3T3 cells from oxidant-induced cell death. |
| Keywords: | Apoptosis Free radical scavenging Lipid peroxidation Mangifera indica Mangiferin Pro-oxidant Apoptosis Oxidant-induced cell death |
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