Some Characteristics of Free Cell Population in the Airways of Rats after Intratracheal Instillation of Copper-Containing Nano-Scale Particles |
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| Authors: | Larisa I Privalova Boris A Katsnelson Nadezhda V Loginova Vladimir B Gurvich Vladimir Y Shur Yakov B Beikin Marina P Sutunkova Ilzira A Minigalieva Ekaterina V Shishkina Svetlana V Pichugova Ludmila G Tulakina Svetlana V Beljayeva |
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| Affiliation: | 1.The Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov Str., Ekaterinburg 620014, Russia; E-Mails: (L.I.P.); (N.V.L.); (V.B.G.); (M.P.S.); (I.A.M.);2.The Institute of Natural Sciences, the Ural Federal University, Ekaterinburg 620000, Russia; E-Mails: (V.Y.S.); (E.V.S.);3.The City Clinical Diagnostics Centre, 28 Dekabristov Str., Ekaterinburg 620142, Russia; E-Mails: (Y.B.B.); (S.V.P.); (L.G.T.); (S.V.B) |
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| Abstract: | We used stable water suspensions of copper oxide particles with mean diameter 20 nm and of particles containing copper oxide and element copper with mean diameter 340 nm to assess the pulmonary phagocytosis response of rats to a single intratracheal instillation of these suspensions using optical, transmission electron, and semi-contact atomic force microscopy and biochemical indices measured in the bronchoalveolar lavage fluid. Although both nano and submicron ultrafine particles were adversely bioactive, the former were found to be more toxic for lungs as compared with the latter while evoking more pronounced defense recruitment of alveolar macrophages and especially of neutrophil leukocytes and more active phagocytosis. Based on our results and literature data, we consider both copper solubilization and direct contact with cellular organelles (mainly, mitochondria) of persistent particles internalized by phagocytes as probable mechanisms of their cytotoxicity. |
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| Keywords: | ultrafine copper-containing particles bronchoalveolar lavage cytotoxicity |
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