罗汉果提取物的抗氧化活性研究

目的:研究了不同罗汉果提取物体外抗氧化活性。方法:采用D-脱氧核糖法,抗超氧阴离子试剂盒法及比色法分析了不同罗汉果提取物清除自由基及抑制大鼠红细胞自氧化溶血和丙二醛(MDA)的生成。结果:(1)罗汉果提取物(MGC、MGI、MGII)均能有效地清除自由基及减少红细胞溶血的发生,但随抗氧化体系的不同,不同的罗汉果提取物的抗氧化活性差异较大。(2)罗汉果皂甙是罗汉果提取物中抑制红细胞自氧化溶血及丙二醛(MDA)生成的主要的活性成分,它与提取物中其它组分具有清除羟基自由基和O2·的协同作用。结论:罗汉果提取物具有抗氧化活性,罗汉果皂甙是提取物中主要的抗氧化活性成分。

Study on in vitro Antioxidant Activity of Extracts from Siraitia grosvenori. Fruits

Objective: Study on antioxidant activity of different extracts from fruits of Siraitia grosvenori. in vitro. Methods: D-deoxyribose method, reagent box method and spectrophotometry method were used to determine the inhibiting effect on radical activity and hemolysis of red blood cells. Results: 1. Different extracts from fruits of Siraitia grosvenori. all had inhibiting effects on hydroxyl radical (·OH) activity, superoxide anion radical (O·2)activity, MDA and the hemolysis of red blood cells. But antioxidant effects of different extracts were different in different antioxidant systems. 2. Mogrosides were the main components that inhibit MDA and hemolysis of red blood cells, and also had synergetic effect with the other components of extracts on scavenging ·OH and O·2 radicals. Conclusion: Extracts from fruits of Siraitia grosvenori. had antioxidant effects while Mogrosides were the main antioxidant components of the extracts.