沙枣种子总酚的提取及纯化

目的：研究沙枣种子总酚的提取及纯化工艺。方法：采用单因素试验和L9(34)正交试验法，筛选最佳提取工艺，并考察大孔吸附树脂法和离子沉淀法对总酚粗取物的纯化工艺。结果：沙枣种子总酚的最佳制备工艺为：样品加30 倍量40% 甲醇溶液回流提取3 次，每次1h，减压浓缩，得总酚粗提物。粗提物上AB-8 大孔吸附树脂柱，用水洗至流出液无糖后，再用2 倍柱体积的40% 乙醇洗脱，收集流份，浓缩干燥即得。结论：该制备工艺提取纯化沙枣总酚效果较好，工艺的重复性和稳定性好，制备的3 批提取物中总酚平均含量为52.17%。

Extraction and Purification of Total Polyphenols from Elaeagnus angustifolia Seeds

Objective: To study the respective processes for the extraction and purification of total polyphenols from Elaeagnus angustifolia seeds. Methods: Mixtures of methanol and water at different volume ratios were used to extract total polyphenols from Elaeagnus angustifolia seeds, and 40% aqueous methanol solution was selected. Reflux extraction presented higher both yield and purity of total polyphenol extract when compared to ultrasonic extraction and shaking extraction. Subsequently, orthogonal array design method was used to optimize the process for the reflux extraction of total polyphenols using 40% aqueous methanol solution. In the final section of this study, the purification of the crude total polyphenol extract from Elaeagnus angustifolia seeds was investigated by macroporous resin adsorption or ionic precipitation. Results: The optimal extraction process of total polyphenols from Elaeagnus angustifolia seeds was based on the use of 30-fold volume of 40% aqueous methanol solution to extract the material three times for 1 h each time, followed by vacuum condensation. Macroporous resin adsorption was found more suitable for the purification of the crude total polyphenol extract from Elaeagnus angustifolia seeds than ionic precipitation. An optimal purification was achieved through the steps of adsorption of the target products onto AB-8 resin, washing with distilled water until no saccharides could be detected in the eluate, elution with 2-fold bed volume of 40% aqueous methanol solution, harvesting of eluate fractions, condensation and drying. The preparation of total polyphenols from Elaeagnus angustifolia seeds was performed in triplicate, and an average value of product purity of 52.17% was obtained. Conclusion: The preparation method integrating the above optimized extraction and purification processes has the benefits of good repeatability and stability..