沿海地区紫菜中总砷含量及砷形态分析

建立高效液相色谱-氢化物发生-原子荧光光谱(HPLC-HG-AFS)联用法分析紫菜中4种砷的形态。在优化仪器参数的基础上,采用正交试验法分析样品前处理中的酸浓度、萃取温度和超声时间对砷提取量的影响。当硝酸浓度0.15 mol/L、萃取温度80℃、超声时间1 h时,萃取效果最佳。对低、中、高3个水平进行方法验证,砷形态的回收率为84.0%~113.6%,精密度为1.3%~4.6%,结果显示,沿海地区的紫菜产品中总砷含量高,但有毒砷占比小。方法快速高效,可用于测定紫菜中砷形态的含量。

Analysis of Total Arsenic Content and Arsenic Species in Laver in Coastal Area

A method for high performance liquid chromatography coupled with hydride generation-atomic fluorescence spectrometry was established to analyze the morphology of four arsenic species in laver.On the basis of optimizing instrument parameters,the orthogonal test design method was used to analyze the effect of sample pretreatment acid concentration,extraction temperature and ultrasonic time on arsenic extraction.When the concentration of nitric acid was 0.15 mol/L,the extraction temperature was set to 80℃,and the ultrasonic time was 1 h,the extraction effect was the best.The method was validated at three levels:low,medium and high.The recovery of arsenic was 84.0%~113.6%,and the precision was 1.3%~4.6%.The total arsenic content in seaweed products in coastal areas was high,but the proportion of toxic arsenic was small.The method was fast and efficient,which can be used to determine the content of arsenic in laver.