高效液相色谱法同时测定保健食品中11种功效成分

建立高效液相色谱同时测定保健食品中11种功效成分的高通量分析方法。样品前处理采用甲醇-水-磷酸溶液提取,对油脂样品则采用甲醇-水-磷酸溶液与正己烷液液萃取除脂。采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),乙腈-2 g/L辛烷磺酸钠溶液(加入1 m L磷酸)作为流动相,流速1.0 m L/min,梯度洗脱,用二极管阵列检测器串联荧光检测器检测,外标法峰面积定量。VB2在0.25~25 mg/L、VB6在0.5~20 mg/L、烟酸、烟酰胺在1~100 mg/L、其余5种功效成分在0.5~50 mg/L质量浓度范围内线性良好,相关系数r为0.999 1~1.000 0。在样品中待测成分含量约0.5、1倍和2倍3个水平的添加回收率为86.4%~110%之间,相对标准偏差为0.21%~11.7%。方法的定量限:烟酸、烟酰胺为20 mg/kg,其余9种功效成分方法的定量限为10 mg/kg。

Simultaneous Determination of 11 Functional Components in Health Foods by High Performance' Liquid Chromatography

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination
of 11 functional components in health foods. Samples were extracted with methanol-water-phosphoric acid solution/
n-hexane-methanol-water-phosphoric acid solution (for lipid-containing samples). Analysis was performed using an HPLC
system equipped with an Agilent TC- C18 column (250 mm × 4.6 mm, 5 μm) and a PDA/FLR detector with a mobile phase
composed of acetonitrile and 2 g/L sodium octanesulfonate solution (containing 1 mL of phosphoric acid) at a flow rate of
1.0 mL/min with gradient elution. Good linearity was observed in the range of 0.25–25 mg/L for vitamin B2, 0.5–20 mg/L
for vitamin B6, 1–100 mg/L for nicotinic acid and nicotinamide, 0.5–50 mg/L of the other five functional components with
correlation coefficients of 0.999 1–1.000 0. The average recoveries in health foods were in the range of 86.4%–110% at three
spiked levels with relative standard deviations varying between 0.21% and 11.7% (n = 6). The limit of quantification was
20 mg/kg for nicotinic acid and nicotinamide, and 10 mg/kg for the remaining nine functional components.