大孔树脂对荷叶黄酮的分离纯化

荷叶富含黄酮,可加以开发利用。为了得到新的黄酮制备工艺,以荷叶为原料,采用超滤法处理荷叶乙醇浸提液,去除大分子量物质。选用HZ系列四种新型大孔吸附树脂,采用静态吸附试验对大孔吸附树脂进行筛选。以超滤液作为样品液,对筛选得到的吸附树脂,用动态吸附解吸试验选择优化了吸附解吸操作条件。结果表明,HZ-806大孔吸附树脂对荷叶黄酮的吸附性能与解吸效果最好。选定HZ-806的吸附条件为:上柱液pH4.0,上柱液浓度2.0mg/mL,上柱液体积10BV(BV为树脂柱体积)、上柱流速2BV/h。洗脱条件为:乙醇浓度60%,洗脱流速1BV/h,洗脱液体积为3.5BV。在上述优化条件下经过超滤处理过的上柱液从吸附到解吸操作荷叶黄酮总得率96.85%。荷叶黄酮含量从27.06%提高到61.91%,其中占总黄酮的91.98%洗脱组分,纯度达到81.58%;HPLC检测表明荷叶黄酮中芦丁上柱前后含量从7.03%提高到27.93%。说明该工艺是获取荷叶黄酮有效精制、分离方法。

Isolation and Purification of Flavonoids from Lotus Leaf by Macroporous Resins

A method for isolating and purifying flavonoids from lotus-leaf was developed in this study.Macromolecular compounds in the crude flavonoids extracted by ethanol from lotus-leaf were removed by ultra-filtration.Four kinds of HZ-series macroporous resins were used for the isolation and purification of the ultra-filtrate by static adsorption.And the purification conditions were optimized by dynamic adsorption test.Among the examined resins,HZ-806 resin was shown to be the best due to its highest adsorption and desorption properties,with which the optimum adsorption conditions were found to be flavonoids of 2.0 mg/mL,ratio of flavonoid volume to the bed volume of 10BV,pH of 4.0,and flow rate of 2 BV/h,while the optimal desorption conditions were found to be a flow rate of 1 BV/h,ratio of flavonoid volume to the bed volume of 3.5 BV and ethanol of 60%.Under those conditions,lutein content greatly increased from 7.0% to 27.9% after the column purification,and the content of lotus-leaf flavonoids increased from 27.06% to 61.91% with a total recovery of flavonoids being of 96.85%.And the purity of a main component(91.98%) in the total flavonoids was 81.58%.