罗非鱼皮Ⅰ型胶原蛋白的抗原反应特性分析

目的：确定罗非鱼皮Ⅰ型胶原蛋白的抗原性，为进一步研究罗非鱼皮Ⅰ型胶原蛋白生物相容性提供依据。方法：采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electrophoresis，SDS-PAGE）对分离提取的罗非鱼皮胶原蛋白进行鉴定，共免疫ICR小鼠3 次。采用酶联免疫吸附反应（enzymelinked immune sorbent assay，ELISA）分别于每次免疫后第7天测定小鼠产生的总胶原蛋白抗体，并在第21天测定小鼠血清中内免疫球蛋白G（immunoglobulin G，IgG）、免疫球蛋白A（IgA）和免疫球蛋白M（IgM）的含量。结果：罗非鱼皮胶原蛋白样品是Ⅰ型胶原蛋白。所有经罗非鱼皮Ⅰ型胶原蛋白免疫的小鼠体内均产生抗体，3 次免疫后罗非鱼皮Ⅰ型胶原蛋白抗体质量浓度范围为160.50～164.25 μg/L，小鼠IgG、IgA和IgM的质量浓度范围分别为424.81～437.59 ng/mL、46.86～49.53 μg/mL、1.81～1.89 ng/mL。结论：罗非鱼皮Ⅰ型胶原蛋白对ICR小鼠呈现较弱的抗原性。

Antigen Response Properties of Tilapia Skin Type I Collagen

Objective: To investigate the antigenicity of type I collagen isolated from tilapia skin and evaluate its
biocompatibility. Methods: The purity of tilapia skin collagen was confirmed by SDS-PAGE, and the antigenicity was
evaluated by measuring the changes in serum IgG, IgA and IgM induced by tilapia skin type I collagen in ICR mouse model
using ELISA. Results: The tilapia skin type I collagen was pure. The antibody concentrations of tilapia skin collagenimmunized
mice were 160.50–164.25 μg/L, and the concentrations of IgG, IgA and IgM were 424.81–437.59 ng/mL,
46.86–49.53 μg/mL and 1.81–1.89 ng/mL, respectively. Conclusion: Tilapia skin type I collagen has weak antigenicity
responsitivty and proved to have superior biocompatibility for biomedical applications.