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| 基于PMT的激光共聚焦生物芯片微弱荧光检测 | |
| 引用本文： | 余勇,胡松. 基于PMT的激光共聚焦生物芯片微弱荧光检测[J]. 微纳电子技术, 2006, 43(1): 50-54 |
| 作者姓名： | 余勇 胡松 |
| 作者单位： | 1. 中科院光电技术研究所,成都,610209;中国科学院研究生院,北京,100039 2. 中科院光电技术研究所,成都,610209 |
| 基金项目： | 中国科学院知识创新工程项目 |
| 摘 要： | 介绍了激光共聚焦扫描仪的成像原理以及PMT的特性和控制原理,在此基础上详细分析了FPGA通过DAC动态控制PMT增益的微弱荧光信号检测方法,以适应不同的荧光强度,FPGA同时控制16位高速AD转换,得到高精度图像数据。实验结果表明,系统具有高精度和高分辨率的特点,可探测到0.1个荧光分子/μm2。 |
| 关 键 词： | 生物芯片 共聚焦 光电倍增管 荧光 现场可编程门阵列 |
| 文章编号： | 1671-4776(2006)01-0050-05 |
| 收稿时间： | 2005-07-12 |
| 修稿时间： | 2005-07-12 |
Laser Confocal Biochip Scanner Based on PMT |
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| YU Yong,HU Song. Laser Confocal Biochip Scanner Based on PMT[J]. Micronanoelectronic Technology, 2006, 43(1): 50-54 | |
| Authors: | YU Yong HU Song |
| Affiliation: | 1.Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China; 2.Graduate School of Chinese Academy of Sciences, Belting 100039, China |
| Abstract: | The imaging principle of the laser confocal biochip scanner,the property and control principle of PMT are introduced. And on the base of the above discussion,the weak fluorescent detection that the gain of the PMT controlled by FPGA through digital to analog converter(DAC)dynamically to adapt different fluorescent intensity is analyzed. The high speed 16 bit analog to digital converter(AD)is also controlled by FPGA,and high precision image data is obtained. The experiments prove that it can detect 0.1 fluorophor/μm2,the system is high in precision and resolution. |
| Keywords: | biochip confocal PMT fluorescent FPGA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |