|
|||||
|
|
| N-乙基-N-丁基吗啉离子液体双水相体系萃取分离蛋白质 | |
| 引用本文： | 王军,张艳,时召俊,范必威. N-乙基-N-丁基吗啉离子液体双水相体系萃取分离蛋白质[J]. 应用化工, 2009, 28(1) |
| 作者姓名： | 王军 张艳 时召俊 范必威 |
| 作者单位： | 1. 成都理工大学,材料与化学化工学院,四川,成都,610059 2. 河南大学,生命科学学院,河南,开封,475002 3. 安徽八一化工股份有限公司,安徽,蚌埠,233000 |
| 摘 要： | 研究了新型离子液体N-乙基-N-丁基吗啉四氟硼酸盐([Nebm]BF4)和KH2PO4形成的双水相体系对牛血清白蛋白(BSA)的萃取行为,考察了盐的加入量、离子液体浓度、溶液pH值、蛋白质浓度等因素对萃取率的影响。结果表明:当KH2PO4的加入量为85 g/L、离子液体浓度在200~250 g/L、BSA的浓度60~120 mg/L、溶液酸度在pH4.5~7.0时,其萃取率达98.0%以上。该双水相体系对α-淀粉酶的萃取率也达98.5%。 |
| 关 键 词： | 离子液体 双水相体系 萃取 蛋白质 |
Separation of BSA in N-ethyl-N-butylmorpholine tetrafluoroborate ionic liquid aqueous two-phase system |
|
| WANG Jun,ZHANG Yan,SHI Zhao-jun,FAN Bi-wei. Separation of BSA in N-ethyl-N-butylmorpholine tetrafluoroborate ionic liquid aqueous two-phase system[J]. Applied chemical industry, 2009, 28(1) | |
| Authors: | WANG Jun ZHANG Yan SHI Zhao-jun FAN Bi-wei |
| Abstract: | A new method to extract and separate bovine serum albumin(BSA) in aqueous two-phase system(ATPS) formed by a room temperature water-soluble ionic liquid N-ethyl-N-butylmorpholine tetrafluoroborate( BF4) and phase-forming salt KH2PO4 was studied.The effects of the concentration of salt,ionic liquid and BSA,solution pH value on extraction efficiency were investigated systematically.The results showed that the solution pH value 4.5～7.0,BF4 200～250 g/L and KH2PO4 85 g/L,the extraction efficiency was 98.0%,and the extraction efficiency of α-amylase was more than 98.5%. |
| Keywords: | ionic liquids ATPS extraction protein |
| 本文献已被 维普 万方数据 等数据库收录！ | |