菟丝子中总黄酮的闪式提取工艺及近红外透射光谱含量分析方法

利用闪式提取器对菟丝子中的总黄酮进行组织破碎提取,确定菟丝子中总黄酮闪式提取的最佳提取工艺为:料液比1∶8(mg∶mL),以50%的乙醇为溶剂提取8min。采用主成分分析(PCA)和偏最小二乘法(PLS)回归分析法建立了菟丝子提取液中总黄酮含量近红外透射光谱定量分析模型,该模型校正集的相关系数为0.99985,校正集标准偏差(RMSEC)为0.0685,预测集标准偏差(RMSEP)为0.160,交叉验证结果的相关系数为0.99966,交叉验证标准差(RMSECV)为0.102。利用该模型预测和紫外分光光度法测量的总黄酮含量相差很小,且模型具有良好的稳定性。

Study on Flash Extraction Process of Total Flavonoids from Cuscuta Chinensis and Its Content Determination by Near Infrared Transmittance Spectroscopy

The smashing tissue extraction of total flavonoids from Cuscuta chinensis was conducted in flash extractor.The optimum flash extraction conditions were as follows:ethanol concentration 50%,ratio of material to liquid 1∶8(mg∶mL),extraction time 8 min.Based on the principal component analysis(PCA) and partial least squares(PLS) regression analysis,a quantative analysis model NIRS(near infrared transmittance spectroscopy) was established for the content determination of total flavonoids from Cuscuta chinensis,for which the calibration correlation coefficient was 0.99985,the calibration standard deviation was 0.0685,the prediction standard deviation was 0.160,and the correlation coefficient was 0.99966 by cross-validation,the prediction standard deviation by cross-validation was 0.102.The total flavonoids content predicted by the model was very close to that determined by the UV spectrophotometry.The results also indicated that the prediction model had good stability.