凝胶层析和离子交换层析结合法纯化重组降血压肽VLPVPR

为了提高VLPVPR的纯度,采用凝胶层析结合离子交换层析的方法对VLPVPR进行纯化。先用Sephadex G-10凝胶对VLPVPR溶液脱盐,再考察离子交换剂（SP Sepharose Fast Flow和Q Sepharose Fast Flow）对重组降血压肽VLPVPR的纯化效果,确立纯化VLPVPR的优化工艺。实验结果表明SP Sepharose Fast Flow不适于VLPVPR的纯化。采用Q Sepharose Fast Flow的纯化工艺为:上样量为柱床体积的10%,用10mmol/L pH9.0的CHES缓冲液洗脱,流速为1mL/min。VLPVPR回收率为93.8%,纯度达到47.2%。采用Q Sepharose Fast Flow纯化提高了VLPVPR的纯度。 

Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography

To purify the peptide VLPVPR, gel filtration combined with ion exchange chromatography method was employed.Sephadex G-10 was used to desalt firstly.Compared the purification effective of SP Sepharose Fast Flow and Q Sepharose Fast Flow.SP Sepharose Fast Flow was not suitable for VLPVPR purification while Q Sepharose Fast Flow was suitable for VLPVPR purification.The optimization condition of Q Sepharose Fast Flow were pH9.0, feeding volume 10% BV, CHES concertration 10 mmol /L, elute rate 1.0mL /min.The recovery ratio was 93.8% and the purity ratio was 47.2%.The purification effective of Q Sepharose Fast Flow was improved.