BAF反应器中好氧反硝化菌的分子鉴定及分析

从处理高浓度蛋白质废水的BAF反应器中经富集分离出一株能以KNO3为唯一氮源生长的好氧反硝化菌N1。为进一步研究其分子生物学特性,采用传统的生理生化特征鉴定法及16SrDNA序列分析法对该菌株进行研究,并与已知种和相关种的菌株进行比较,得到系统发育树状图。结果表明,菌株N1的16SrDNA的核苷酸序列与蒙氏假单胞菌（Pseudomonas monteilii strainCIP 104883）的同源性为99.2%,在细菌系统发育分类学上属于假单胞菌属,蒙氏假单胞菌。好氧反硝化菌株N1的序列已向GenBank提交并得到登录号为HQ840771。关于蒙氏假单胞菌的反硝化研究在国内尚未见报道,此研究对于利用微生物技术治理环境中的氮污染具有较高的研究和应用价值。

Molecular Classification, Identification and Phylogenetic Analysis of Aerobic Denitrifier N1 in the BAF Reator

A strain of aerobic denitrifier N1 screened from the biofilm of BAF combined reactor for treating high concentration organic pharmaceutical wastewater was found capable of aerobic denitrification,which can be used nitrate of potash by as sole nitro-gen source.The results indicated that the strain N1 was most similar to Pseudomonas monteilii based on the results of morphologic characteristics,physiological and biochemical properties and phylogentic analysis of 16S rDNA sequence,and the sequence had the highest similarity of 99.2% with 16S rDAN sequence of strain Pseudomonas monteilii strain CIP 104883 obtained from GenBank using BLAST.At present,there are few reports on the degradation of nitrate nitrogen with Pseudomonas monteilii.The nucleotide sequences of strain N1 have been submitted to the GenBank databases under accession numbers HQ840771.So far there are few studies related to aerobic denitrificaton of Pseudomonas monteilii.Therefore,the study is high valuable for treating nitrogenous wastewater using microbe technology.