一株节杆菌脱硫特性的初步研究

从油污染的土壤样品中筛选出一株能将二苯并噻吩（DBT）代谢为2-羟基联苯（2-HBP）的脱硫菌株T1，初步鉴定其属于节杆菌属（Arthrobacter.sp）。在0.5mmol/L DBT浓度下,菌株具有较高的脱硫活性；相对于硫酸钠、二甲基亚砜为唯一硫源，T1菌株更容易利用DBT；在硫酸钠初始浓度大于0.5mmol/L时几乎无2-HBP生成；在2-HBP初始浓度大于0.5mmol/L时对T1的生长有明显的抑制作用。

BIODESULFURIZATION OF ORGANIC SULFUR BY A NEWLY ISOLATED BACTERIAL STRAIN T1

A kind of bacterial strain, T1, having the function of converting dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP) was cultured and isolated from crude oil contaminated soil, and it was preliminarily identified as a member of Arthrobacter.sp. Strain. At a DBT concentration of 0.5 mmol/L, T1 bacterial strain exhibited high desulfurization activity. As compared with using sodium sulfate or dimethyl sulphoxide as the single sulfur source, when using DBT T1 was easy to be cultured and to exhibit its sulfur converting function. The existence of sodium sulfate and 2-HBP strongly suppressed the growth of T1 and its desulfurization activity, e.g. only a few 2-HBP was generated by strain in culture with a sodium sulfate initial concentration of 0.5 mmol/L, and the cell–growth of T1 slowed down significantly when the 2-HBP concentration reached 0.5 mmol/L.