高效液相色谱-荧光法测定油炸型膨化食品中4种多环芳烃

建立了高效液相色谱-荧光法(HPLC-FLD)测定油炸型膨化食品中苯并a]蒽(BaA)、(CHR)、苯并b]荧蒽(BbF)、苯并a]芘(BaP)4种多环芳烃(PAHs)的分析方法。样品经饱和氯化钠分散后,采用正己烷萃取,经BAP-3分子印迹柱净化,Waters PAH C₁₈色谱柱分离,荧光检测器进行测定,外标法定量。在0.5~40 μg/L浓度范围内4种PAHs均有良好的线性(r>0.999),定量限为(LOQ)0.3~0.5 μg/kg。在空白样品中进行1、5、25 μg/kg 3个水平的加标回收实验,方法的平均回收率在90.3%~107.4%之间,精密度在6.5%以下。该方法简单、快速、灵敏、重现性好,可用于油炸型膨化食品中4种PAHs的定量测定。

Determination of Four Polycyclic Aromatic Hydrocarbons in Fried Expanded Food by High Performance Liquid Chromatography-Fluorescent Detection

A method was developed for the determination of four kinds of polycyclic aromatic hydrocarbons(PAHs)including benzoa]anthracene(BaA),chrysene(CHR),benzob]fluoranthene(BbF),benzoa]pyrene(BaP)in fried expanded food by high performance liquid chromatography-fluorescent detection(HPLC-FLD). The samples were dispersed with saturated sodium chloride solution,extracted with n-Hexane and cleaned-up by BAP-3 molecularly imprinted solid phase extraction column. The products were separated on a Waters PAH C₁₈column and analyzed by fluorescence detector with external standard method. Under the optimal conditions,four kinds of PAHs showed good linearity(r>0.999)in range from 0.5~40 μg/L. The limits of quantitation(LOQ)of four kinds of PAHs were 0.3~0.5 μg/kg. The spiked recoveries of analytes from blank sample at three levels of 1,5 and 25 μg/kg were in the range of 90.3%~107.4% with the relative standard deviations less than 6.5%. With the advantages of convenience,fast,sensitivity and good repeatability,the method was suitable for the detection of four kinds of PAHs in fried expanded food.