Candida molischianaβ-Glucosidase Production by Saccharomyces cerevisiae and its Application in Winemaking |
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| Authors: | S Genovés JV Gil P Manzanares JL Aleixandre S Vallés |
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| Affiliation: | Authors Genovés, Gil, Manzanares, and Vallés are with Dept. de BiotecnologíadeAlimentos, Inst. deAgroquímicay TecnologíadeAlimentos, Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 73, 46100 Burjassot, Valencia, Spain. Author Aleixandre is with the Dept. de Tecnología deAlimentos, Univ. Politécnica de Valencia, Valencia, Spain. Direct inquiries to author Vallés (E-mail: ). |
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| Abstract: | ABSTRACT: A recombinant Saccharomyces cerevisiae wine yeast strain expressing the Candida molischiana bgln gene encoding a β-glucosidase (BGLN) has been used to produce this enzyme. Shaking rate, pH, and aeration rate conditions have been optimized to obtain maximum activity to facilitate enzyme purification. The ability of the heterologous enzyme to efficiently release terpenols and alcohols from a Muscat wine glycoside extract and also directly from wine has been demonstrated. Terpenol glycoside content decreased by 50% after 1 mo of wine storage in agreement with results reported for the β-glucosidase produced by C. molischiana. |
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| Keywords: | β-glucosidase Candida molischiana Saccharomyces cerevisiae heterologous production wine aroma |
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