激活素A对体外培养小鼠胎鼠大脑皮层神经元存活的促进作用

目的研究激活素A(Activin A)对体外培养小鼠胎鼠大脑皮层神经元存活的促进作用。方法原代培养孕期17d小鼠胎鼠脑神经细胞,免疫细胞化学染色观察激活素受体(ActRIIA)在神经细胞中的表达;将原代培养神经细胞分为阴性对照组(5%胎牛血清-DMEM/F12培养基)、阳性对照组(4ng/ml神经生长因子)和激活素A(5ng/ml)组,分别于培养第1、3、5、7和9天,采用台酚蓝染色法检测神经元存活情况。结果培养的小鼠胎鼠脑神经细胞可表达ActRIIA;阴性对照组存活神经元呈时间依赖性减少,在培养第9天已检测不到存活的神经元;而阳性对照组和激活素A组在培养第9天仍有部分神经元存活。结论激活素A具有维持小鼠胎鼠大脑皮层神经元长期存活的作用。

Activin A Promotes Survival of Fetal Mouse Cortex Neurons Cultured In Vitro

Objective To investigate the promoting effect of activin A on the survival of fetal mouse cortex neurons cultured in vitro.Methods Seventeen-day-old fetal mouse brain nerve cells were cultured preliminarily,in which the expression of ActRⅡA was observed by immunocytochemical staining.The cells were divided into negative control,positive control and activin A groups,and cultured with 5% fetal bovine serum-DMEM /F12 medium,4 ng /ml nerve growth factor(NGF)and 5 ng /ml activin A respectively,in which the survivals of neurons were determined by trypan blue staining on days 1,3,5,7 and 9 after culture.Results ActRⅡA was expressed in the fetal mouse brain nerve cells cultured in vitro.In negative control group,the surviving neurons decreased in a time-dependent mode,and no surviving neurons were detected on day 9 after culture.However,in positive control and activin A groups,partial neurons still survive on day 9 after culture.Conclusion Activin A may maintain the long-term survival of fetal mouse cortex neurons.